Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionic selectivity of volume-sensitive currents in human epithelial cells.

A Rasola1, L J Galietta, D C Gruenert

  • 1Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova, Italia.

Biochimica Et Biophysica Acta
|August 25, 1992
PubMed
Summary

Swelling-activated chloride currents in human epithelial cells show distinct ion selectivity, preferring iodide over other anions. This selectivity mirrors that of depolarization-activated outwardly rectifying chloride channels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caspases shutdown nonsense-mediated mRNA decay during apoptosis.

Cell death and differentiation·2015
Same author

Myotonic dystrophy protein kinase (DMPK) prevents ROS-induced cell death by assembling a hexokinase II-Src complex on the mitochondrial surface.

Cell death & disease·2013
Same author

Chemotherapeutic induction of mitochondrial oxidative stress activates GSK-3α/β and Bax, leading to permeability transition pore opening and tumor cell death.

Cell death & disease·2012
Same author

Generation of SV40-transformed rabbit tracheal-epithelial-cell-derived blastocyst by somatic cell nuclear transfer.

Cell and tissue research·2012
Same author

Gadd45α activity is the principal effector of Shigella mitochondria-dependent epithelial cell death in vitro and ex vivo.

Cell death & disease·2011
Same author

Bactofection of lung epithelial cells in vitro and in vivo using a genetically modified Escherichia coli.

Gene therapy·2008

Area of Science:

  • Cellular physiology
  • Ion channel function
  • Epithelial transport

Background:

  • Swelling-activated chloride currents play crucial roles in epithelial cell volume regulation.
  • Understanding the ion selectivity of these currents is vital for comprehending epithelial function and dysfunction.

Purpose of the Study:

  • To investigate the ion selectivity profile of swelling-activated chloride currents in human epithelial cell lines.
  • To compare the selectivity of these currents with other known chloride channels in epithelial cells.

Main Methods:

  • Electrophysiological recordings were performed on three human epithelial cell lines: 9HTEo-, CFNPE9o- (airway), and T84 (colon).
  • Ion selectivity was determined by measuring relative anion permeabilities using current-voltage relationships under different ionic conditions.

Related Experiment Videos

Main Results:

  • The relative permeability of swelling-activated currents followed the sequence: I- > NO3- > Br- > Cl- > F- > HCO3- > isethionate > aspartate > gluconate > SO4(2-).
  • Iodide (I-) exhibited the highest relative permeability, while sulfate (SO4(2-)) showed the lowest.

Conclusions:

  • Swelling-activated chloride currents in these human epithelial cells display a specific ion selectivity pattern.
  • This selectivity is comparable to that observed in depolarization-activated outwardly rectifying chloride channels, suggesting potential functional similarities.