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Expression of c-kit protein during placental development
K Horie1, J Fujita, K Takakura
1Department of Gynecology, Faculty of Medicine, Kyoto University, Japan.
Biology of Reproduction
|October 1, 1992
Summary
The c-kit protein, a tyrosine kinase receptor, is expressed in the mouse uterus and placenta during development. Its expression suggests a role in placental proliferation and differentiation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- The c-kit proto-oncogene encodes a transmembrane tyrosine kinase receptor.
- c-kit is allelic with the mouse white-spotting locus (W).
- Understanding c-kit's role in placental development is crucial.
Purpose of the Study:
- To investigate the expression and role of c-kit protein during mouse placental development.
- To examine c-kit protein localization in the uterus and placenta at various developmental stages.
Main Methods:
- Avidin-biotin-peroxidase (ABC) method using anti-c-kit monoclonal antibody.
- Northern blotting.
- Reverse transcriptase-polymerase chain reaction (RT-PCR).
Main Results:
- c-kit protein detected in glandular epithelium (Days 3-5), stromal cells (Day 7), and decidua basalis (Days 9-10).
- In mature placenta, c-kit protein found in labyrinthine and decidual layers.
- c-kit mRNA detected during peri-implantation and placental development stages.
Conclusions:
- c-kit protein expression patterns suggest its involvement in placental development.
- The findings indicate a potential role for c-kit in the proliferation and differentiation of placental cells.