Carcinogenic effects of skin transplantation and immunosuppression in mice

C3H and BlD2 mice received skin transplants from an animal of the other strain. Three groups were immunosuppressed for varying periods of time, and with different doses of vinblastine, cyclophosphamide, or azathioprine. Following transplantation, control animals received daily injections of NaCl. All animals developed tumours of lymphoreticular origin, which would indicate that immunosuppression is only a secondary cause for tumor development, with antigenic stimulation being the primary factor.