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Updated: Aug 14, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Polyacrylamide gel electrophoresis in vertical, inverse and double-crossing gradients of soluble polymers
1Department of Pathology, Georgetown University Medical Center, Washington, DC 20007.
Abstract:
The presence of soluble dextrans, methylcellulose and polyethylene glycol polymers incorporated into vertical sodium dodecyl sulfate (SDS)-polyacrylamide slabs during electrophoresis can have a pronounced effect on protein separations. The effects of various standard and inverse gradients of polymers on the electrophoretic mobility of marker proteins in 10% T, 2.66% C Laemmli-style SDS gels, and the effects of simultaneous pore size and polymer gradients were investigated. These experiments demonstrate that the inclusion of polymers is a new, additional parameter that can be useful in resolving complex mixtures of proteins.
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