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CD5 monoclonal antibodies react with human peripheral blood dendritic cells
1Department of Dermatology, Case Western Reserve University, New Haven, Connecticut.
The American Journal of Pathology
|October 1, 1992
Summary
Human peripheral blood dendritic cells (PBDC) express the CD5 molecule, distinguishing them from monocytes. This finding offers new insights into PBDC activation pathways and immune cell signaling.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD5 is a 67-kDa glycoprotein found on thymocytes, T cells, and some B cells.
- CD5 acts as a signaling molecule, influencing cellular activation thresholds.
- Human peripheral blood dendritic cells (PBDC) are distinct from monocytes and other immune cells.
Purpose of the Study:
- To investigate the expression of CD5 on human peripheral blood dendritic cells (PBDC).
- To determine if PBDC share CD5 expression with other immune cell types.
Main Methods:
- Utilized anti-CD5 monoclonal antibodies (MAbs) Leu-1 and UCHT2.
- Analyzed PBDC, Langerhans' cells, interdigitating cells, monocytes, and macrophages for CD5 expression.
Main Results:
- A subpopulation of PBDC reacted positively with anti-CD5 MAbs.
- Langerhans' cells, interdigitating cells, monocytes, and macrophages were uniformly CD5-negative.
- This indicates that PBDC can express the CD5 molecule.
Conclusions:
- PBDC express the CD5 molecule, differentiating them from monocytes.
- CD5 expression on PBDC provides a new characteristic for distinguishing them from other mononuclear leukocytes.
- Findings may offer insights into PBDC activation pathways.