Related Experiment Videos
In vitro synthesis of bacterio-opsin: integration into microsomal membranes
U Bauer1, V Hildebrandt, N A Dencher
1Freie Universität Berlin, FB Physik, AG Biophysik, Germany.
Biochemical and Biophysical Research Communications
|September 30, 1992
Abstract:
The translation and membrane integration of bacterio-opsin from Halobacterium salinarium were investigated. Plasmids containing the bacterio-opsin-gene with or without its original presequence were transcribed with the T7-RNA-polymerase and translated in vitro in a wheat germ system. The integration of the expressed bacterio-opsin into dog pancreas microsomes was studied. Both precursor bacterio-opsin and mature bacterio-opsin integrate into the eukaryotic membrane.