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A comparison of two autoradiographic methods for detecting radiolabeled nucleic acids in embryos
L Plante1, J W Pollard, W A King
1Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
Molecular Reproduction and Development
|October 1, 1992
Abstract:
Two methods for preparing embryos for autoradiographic study of newly synthesized nucleic acids are described and compared. The first method consists of rapidly fixing radiolabeled embryos with acetic acid:methanol, spreading them on glass slides and exposing them for 8 days with a photographic emulsion. The second method consists of fixing, embedding in resin, and sectioning the embryos before their exposure with the emulsion for 3 weeks. Both techniques have many applications in studies of early embryonic activity, but the spread technique is very sensitive, simpler, and faster.