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Related Experiment Videos

Induction of two K+ currents by complement component C5a in mouse macrophages.

M Ichinose1, N Hara, M Sawada

  • 1Department of Physiology, Shimane Medical University, Izumo, Japan.

Biochimica Et Biophysica Acta
|November 9, 1992
PubMed
Summary

Complement component C5a activates two distinct potassium currents in mouse macrophages. One current is calcium-dependent, while the other is not, suggesting complex signaling pathways.

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Area of Science:

  • Immunology
  • Cellular Physiology
  • Molecular Biology

Background:

  • Complement component C5a (C5a) is a potent anaphylatoxin involved in immune responses.
  • Macrophages play a crucial role in innate and adaptive immunity.
  • Ion channel activity is critical for macrophage function.

Purpose of the Study:

  • To investigate the effects of C5a on ion currents in peritoneal macrophages.
  • To characterize the types of ion currents activated by C5a.
  • To elucidate the ionic and molecular mechanisms underlying C5a-induced currents.

Main Methods:

  • Peritoneal macrophages were isolated from thioglycerol-stimulated mice.
  • Macrophage ion currents were measured using patch-clamp electrophysiology.

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  • Pharmacological agents (quinidine, tetraethylammonium, charybdotoxin) were used to block specific ion channels.
  • Intracellular calcium levels were manipulated using EGTA and BAPTA.
  • Single-channel recordings were performed on cell-attached patches.

    • Main Results:

    • C5a induced two outward currents: a sustained and a transient outward current.
    • Quinidine and tetraethylammonium partially inhibited both currents.
    • Charybdotoxin significantly suppressed the transient outward current.
    • The transient current was independent of extracellular calcium but dependent on intracellular calcium buffering.
    • A C5a-responsive single potassium channel with 29 pS conductance was identified.

    Conclusions:

    • C5a activates distinct calcium-dependent and calcium-independent potassium currents in macrophages.
    • These findings reveal novel roles for C5a in regulating macrophage electrophysiology.
    • The identified potassium channels may be important targets for modulating immune responses.