Related Experiment Video
Updated: Aug 10, 2026

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
Published on: May 19, 2019
Osteoclasts express high levels of p60c-src, preferentially on ruffled border membranes
S Tanaka1, N Takahashi, N Udagawa
1Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Abstract:
Expression of p60c-src, the normal cellular counterpart of the transforming protein of Rous sarcoma virus (RSV), p60v-src, was examined in mouse and rat authentic osteoclasts and mouse osteoclast-like multinucleated cells (MNCs) formed in vitro. In co-cultures of mouse osteoblastic cells and spleen cells, the expression of p60c-src strikingly increased on day 5 in parallel with the appearance of MNCs in the presence of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3). Immunohistochemical examination confirmed the high level expression of p60c-src in both mouse authentic osteoclasts and MNCs. Electron microscopic examination revealed that p60c-src was primarily localized on ruffled border membranes and vacuoles, but not on the clear zone in rat authentic osteoclasts. These results suggest that p60c-src is important in osteoclastic bone resorption.
Related Concept Videos
Cytoskeletal Accessory Proteins
Regulation of Nuclear Protein Sorting
COP Coated Vesicles
Directing Proteins to the Rough Endoplasmic Reticulum
Cell Polarization by Rho Proteins
Osteoclasts in Bone Remodeling

