Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ATP synthase: structure-function relationships.

P J Thomas1, M Bianchet, D N Garboczi

  • 1Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD.

Biochimica Et Biophysica Acta
|July 17, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Mid-Pleistocene Transition: a delayed response to an increasing positive feedback?

Climate dynamics·2023
Same author

The use of surfactant-filled mesoporous silica as an immobilising medium for a fluorescence lifetime pH indicator, providing long-term calibration stability.

RSC advances·2022
Same author

Stochastic oscillators in biology: introduction to the special issue.

Biological cybernetics·2022
Same author

Polycyclic aromatic compounds (PACs) in the Canadian environment: The challenges of ecological risk assessments.

Environmental pollution (Barking, Essex : 1987)·2020
Same author

Polycyclic aromatic compounds (PACs) in the Canadian environment: Exposure and effects on wildlife.

Environmental pollution (Barking, Essex : 1987)·2020
Same author

Mycobacterium tuberculosis ribosomal protein S1 (RpsA) and variants with truncated C-terminal end show absence of interaction with pyrazinoic acid.

Scientific reports·2020
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Researchers are investigating the structure of ATP synthase, focusing on the F1 moiety's alpha and beta subunits and nucleotide binding sites. Current studies utilize X-ray crystallography, chemical, molecular biology, and biochemical methods to understand its function.

Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology
  • Enzyme Mechanisms

Background:

  • The F1 moiety of ATP synthase is crucial for energy production.
  • Understanding the spatial arrangement of alpha and beta subunits is key to elucidating enzyme function.
  • Identifying nucleotide binding domains is essential for comprehending ATP synthesis and hydrolysis.

Purpose of the Study:

  • To review current understanding of the three-dimensional structural relationships within the F1 moiety.
  • To summarize findings on the location of nucleotide binding domains in alpha and beta subunits.
  • To identify challenges in formulating a structure-function model for ATP synthase.

Main Methods:

  • X-ray crystallography to determine atomic-level structures.

Related Experiment Videos

  • Chemical approaches to probe subunit interactions and modifications.
  • Molecular biology techniques to investigate subunit roles and mutations.
  • Biochemical assays to study enzyme activity and binding properties.

    • Main Results:

    • Significant progress has been made in mapping subunit interactions and nucleotide binding sites.
    • Multiple lines of evidence converge on specific structural arrangements critical for function.
    • However, a complete, integrated model remains elusive.

    Conclusions:

    • Current research provides a foundational understanding of ATP synthase's F1 subunit structure.
    • Further integration of data from diverse methodologies is required.
    • Resolving remaining structural and functional questions is necessary for a comprehensive model of ATP synthase.