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Related Experiment Videos

Surface-immobilized polyethylene oxide for bacterial repellence.

N P Desai1, S F Hossainy, J A Hubbell

  • 1Department of Chemical Engineering, University of Texas, Austin 78712-1062.

Biomaterials
|January 1, 1992
PubMed
Summary

Surface modification of polyethylene terephthalate films with polyethylene oxide significantly reduced bacterial adhesion, potentially lowering the risk of implant infections. Plasma fibrinogen was key to bacterial attachment on both modified and unmodified films.

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Infectious Diseases

Background:

  • Implant-associated infections are a significant clinical challenge.
  • Polyethylene terephthalate (PET) is a common material for medical implants.
  • Bacterial adhesion to implant surfaces is the initial step in infection development.

Purpose of the Study:

  • To evaluate the efficacy of surface modification of PET films with polyethylene oxide (PEO) in reducing bacterial adhesion.
  • To investigate the role of plasma proteins, specifically fibrinogen, in bacterial adhesion to modified and unmodified PET surfaces.

Main Methods:

  • Polyethylene terephthalate films were surface-modified with polyethylene oxide (18,500 g/mol) via a solution technique.
  • Three common implant-associated bacteria (Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa) were cultured on modified and control PET films.

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  • Bacterial adhesion was quantified, and the influence of human plasma and serum, containing fibrinogen, was assessed.

    • Main Results:

    • Polyethylene oxide-modified PET films exhibited significant reductions in adherent bacteria (70-95%) compared to untreated PET.
    • Plasma fibrinogen was identified as a crucial factor promoting bacterial adhesion for all tested strains on both PEO-modified and control PET surfaces.

    Conclusions:

    • Surface modification of PET with polyethylene oxide demonstrates a promising strategy to reduce bacterial adhesion and mitigate the risk of implant-associated infections.
    • Understanding the role of plasma proteins like fibrinogen is essential for developing effective anti-adhesion strategies for medical implants.