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Related Experiment Videos

Atomic force microscopy of single- and double-stranded DNA.

H G Hansma1, R L Sinsheimer, M Q Li

  • 1Department of Physics, University of California, Santa Barbara 93106.

Nucleic Acids Research
|July 25, 1992
PubMed
Summary

Researchers developed a new atomic force microscope (AFM) method to image single-stranded DNA (ssDNA). This technique visualizes ssDNA structure and length, offering insights into its physical properties.

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Atomic force microscopy (AFM) is a powerful tool for nanoscale imaging.
  • Visualizing single-stranded DNA (ssDNA) presents unique challenges due to its flexibility and lower structural definition compared to double-stranded DNA (dsDNA).

Purpose of the Study:

  • To establish a reliable method for imaging ssDNA using AFM.
  • To characterize the structural and dimensional properties of ssDNA in AFM imaging.

Main Methods:

  • Imaging of phi X174 ssDNA and lambda dsDNA using AFM.
  • Experiments conducted in propanol, butanol, and air.
  • Comparison of ssDNA and dsDNA morphology in AFM.

Main Results:

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  • Successful imaging of ssDNA in various environments (propanol, butanol, air).
  • Measured ssDNA lengths typically around 1 µm, with some molecules extending to 1.9 µm.
  • ssDNA appeared lumpier than dsDNA in AFM images.
  • AFM revealed sharper kinks in dsDNA than electron microscopy.
  • Hot water treatment de-aggregated dsDNA plasmids.

    • Conclusions:

    • The developed AFM method enables visualization of ssDNA.
    • AFM provides distinct morphological information differentiating ssDNA from dsDNA.
    • This technique advances nanoscale imaging of nucleic acids.