Related Experiment Videos
Increased phospholipase A2 activity in peritoneal leukocytes in rat experimental colitis
1Krancer Center for Inflammatory Bowel Disease Research, Hahnemann University, Philadelphia, Pennsylvania.
Inflammation
|June 1, 1992
Summary
Peritoneal leukocytes in rats with colitis release more phospholipase A2 (PLA2) and eicosanoids. This suggests leukocytes contribute to inflammation in inflammatory bowel disease.
Area of Science:
- Gastroenterology
- Immunology
- Inflammation Research
Background:
- Activated leukocytes in the peritoneal cavity release phospholipase A2 (PLA2) and eicosanoids.
- The role of peritoneal leukocytes in inflammatory bowel diseases (IBD) remains unclear.
Purpose of the Study:
- To investigate PLA2 activity and eicosanoid production in peritoneal leukocytes during experimental colitis in rats.
- To determine if peritoneal leukocytes contribute to the inflammatory response in IBD models.
Main Methods:
- Induction of experimental colitis in rats using dinitrochlorobenzene, immune complexes, and acetic acid.
- Measurement of peritoneal lavage fluid protein, leukocyte count, PLA2 activity, and eicosanoid concentrations.
- In vitro studies using peritoneal leukocytes and treatment with dexamethasone.
Main Results:
- All colitis models showed increased peritoneal protein, leukocyte numbers, and PLA2 activity (4-32 fold).
- Eicosanoid concentrations were elevated (3-4 fold) in colitis models.
- Peritoneal leukocytes were confirmed as the source of PLA2; dexamethasone reduced PLA2 but not histology.
Conclusions:
- Peritoneal leukocytes are a significant source of PLA2 and eicosanoids in experimental colitis.
- These inflammatory mediators from peritoneal leukocytes may exacerbate inflammation in the colon during IBD.
- Targeting peritoneal leukocyte activity could be a therapeutic strategy for inflammatory bowel diseases.