[Antibiotic sensitivity of Vibrio cholerae 01 isolated from environmental objects]

Area of Science:

• Microbiology
• Environmental Science
• Infectious Diseases

Background:

• Vibrio cholerae-O1 is a significant human pathogen.
• Environmental surveillance is crucial for understanding pathogen dynamics.
• Antibiotic resistance in V. cholerae-O1 poses a public health threat.

Purpose of the Study:

• To assess the antibiotic sensitivity of Vibrio cholerae-O1 strains from environmental sources.
• To characterize the biological properties of these environmental V. cholerae-O1 isolates.
• To determine the prevalence of antibiotic resistance determinants.

Main Methods:

• Isolation and identification of Vibrio cholerae-O1 from environmental objects.
• Antibiotic sensitivity testing using serial dilutions on solid media.
• Characterization of biological properties and detection of resistance determinants.

Main Results:

• High sensitivity observed for tetracycline, chloramphenicol, gentamicin, erythromycin, and rifampicin.
• Lower sensitivity noted for novobiocin, other aminoglycosides, and beta-lactams.
• Resistance determinants were rare (6.3%), with kanamycin and novobiocin resistance found in 15/16 tested strains.

Conclusions:

• Environmental Vibrio cholerae-O1 isolates generally maintain sensitivity to a wide range of antibiotics.
• Changes in biological properties did not consistently correlate with altered antibiotic sensitivity.
• Low prevalence of resistance determinants suggests limited antibiotic pressure in the studied environment.