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[Study of the immunosuppressive effect of cyclosporine in experimental studies]
Abstract:
Specific immunosuppressive activity of cyclosporine prepared at the National Research Centre of Antibiotics by using its original producing culture was studied. It inhibited basic cellular immune responses such as DTH and transplant vs. host and prolonged the lifespan of tumor xenografts under the kidney capsule. Cyclosporine also suppressed the humoral immunity. Its inhibitory effect on lymphocyte blast transformation induced by lectins and in mixed lymphocyte cultures was shown in vitro. Cyclosporine inhibited activity of natural killer cells and T-suppressor cells induced by concanavalin A. It was demonstrated on the models of skin allotransplantation in mice and heterotopic transplantation of the heart in rats that cyclosporine prolonged the transplant lifespan. By the main indices of the cellular immunity and in the models of the transplantation immunity, cyclosporine prepared at the National Research Centre of Antibiotics was shown to be similar to that manufactured by Sandoz.
Insights
Cyclosporine from the National Research Centre of Antibiotics effectively suppressed cellular and humoral immunity, demonstrating comparable immunosuppressive activity to Sandoz cyclosporine in transplantation models.
Area of Science:
- Immunology and Pharmacology
- Biotechnology and Pharmaceutical Research
Context:
- Cyclosporine is a critical immunosuppressive agent.
- Evaluating novel production methods for established drugs is essential.
- The National Research Centre of Antibiotics developed an original cyclosporine-producing culture.
Purpose:
- To assess the specific immunosuppressive activity of cyclosporine produced by the National Research Centre of Antibiotics.
- To compare its efficacy against standard cyclosporine formulations.
Summary:
- The study investigated cyclosporine's immunosuppressive effects, including inhibition of delayed-type hypersensitivity (DTH) and host-versus-graft responses.
- In vitro studies demonstrated suppression of lymphocyte blast transformation and natural killer cell activity.
- In vivo models showed prolonged survival of tumor xenografts and successful prolongation of skin allografts and heart heterotopic transplants.
- The cyclosporine produced exhibited comparable efficacy to Sandoz cyclosporine based on key cellular immunity and transplantation immunity indices.
Impact:
- Validates a new production method for cyclosporine, potentially improving accessibility.
- Provides evidence for the therapeutic potential of domestically produced immunosuppressants.
- Contributes to the understanding of cyclosporine's mechanism of action in various immune responses.