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Glucose and oxygen deprivation induces a Ca(2+)-mediated decrease in (Na(+)+K+)-ATPase activity in rat brain slices.

T Matsuda¹, I Shimizu, Y Murata

¹Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

|April 3, 1992

Summary

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Brain cell energy depletion from lack of glucose or oxygen impairs sodium-potassium pump activity. This crucial enzyme

Area of Science:

Neuroscience
Biochemistry
Cell Physiology

Background:

The sodium-potassium pump ((Na(+)) + K(+))-ATPase is vital for maintaining cellular homeostasis.
Energy deprivation, such as glucose or oxygen deficiency, can significantly impact neuronal function.

Purpose of the Study:

To investigate the effects of glucose and oxygen deprivation on (Na(+)) + K(+))-ATPase activity in rat brain cortical slices.
To explore the role of intracellular calcium and other factors in mediating these effects.

Main Methods:

Rat brain cortical slices were subjected to glucose deprivation, oxygen deprivation, or both.
Measurements included ATP content, (Na(+)) + K(+))-ATPase activity, partial reaction rates, and [3H]ouabain binding.
The influence of intracellular calcium chelators (BAPTA-AM), extracellular calcium, and various drugs was assessed.

Related Experiment Videos

Main Results:

Glucose and oxygen deprivation reduced ATP content and inhibited (Na(+)) + K(+))-ATPase activity and its partial reactions in brain slices.
These inhibitory effects were blocked by BAPTA-AM, indicating a role for intracellular calcium.
Glucose-free hypoxia-induced inhibition was dependent on extracellular calcium and could be modulated by Mg2+, bepridil, and amiloride.

Conclusions:

Energy depletion significantly impairs brain (Na(+)) + K(+))-ATPase function, partly via intracellular calcium.
The kidney (Na(+)) + K(+))-ATPase is notably more resistant to these metabolic insults compared to the brain enzyme.