Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The ovarian contribution to peripherally derived serum C19 conjugates.

R K Matteri1, F Z Stanczyk, D L Cassidenti

  • 1Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles 90033.

The Journal of Clinical Endocrinology and Metabolism
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progesterone and not estrogens or androgens causes breast cancer.

Climacteric : the journal of the International Menopause Society·2024
Same author

Impact of estetrol (E4) on hemostasis, metabolism and bone turnover in postmenopausal women.

Climacteric : the journal of the International Menopause Society·2022
Same author

Hormone replacement therapy - where are we now?

Climacteric : the journal of the International Menopause Society·2021
Same author

Bioidentical hormones.

Climacteric : the journal of the International Menopause Society·2021
Same author

Metabolic and cardiovascular effects of TX-001HR in menopausal women with vasomotor symptoms.

Climacteric : the journal of the International Menopause Society·2019
Same author

Circulating androgen levels before and after oophorectomy in premenopausal and postmenopausal women.

Climacteric : the journal of the International Menopause Society·2019

The ovary does not directly produce C19 conjugates, but ovarian androgens like testosterone and androstenedione influence peripheral androgen metabolism markers. This study investigated the ovarian contribution to these important hormonal conjugates.

Area of Science:

  • Endocrinology
  • Reproductive Biology
  • Metabolism

Background:

  • Serum markers of peripheral androgen metabolism, such as 5 alpha-androstane-3 alpha, 17 beta-diol glucuronide (3 alpha-diolG) and androsterone glucuronide (AoG), are correlated with adrenal androgen production.
  • The relative ovarian contribution to the pool of various C19 conjugates remains under-investigated.

Purpose of the Study:

  • To determine if the ovary directly produces C19 conjugates.
  • To assess the indirect ovarian contribution to C19 conjugate production by examining the effect of ovarian suppression and stimulation on circulating androgen conjugate levels.

Main Methods:

  • Blood samples were collected from ovarian and peripheral veins of normal women.
  • Ovarian suppression and stimulation were performed in normal women and anovulatory nonhirsute patients with polycystic ovary (PCO) using GnRH agonist and gonadotropins.

Related Experiment Videos

  • Unconjugated and conjugated androgens were quantified using radioimmunoassays (RIAs).

    • Main Results:

    • No peripheral-ovarian gradients were found for 5 alpha-androstane-3 alpha, 17 beta-diol sulfates/glucuronides (3 alpha-diolS/3 alpha-diolG) or androsterone sulfates/glucuronides (AoS/AoG).
    • Ovarian suppression significantly decreased AoS and 3 alpha-diolS levels, with decreases in AoS correlating with testosterone (T) suppression in the PCO group.
    • Ovarian stimulation significantly increased T and androstenedione (A) levels, and subsequently, Ao and 3 alpha-diol conjugate levels, particularly AoS and 3 alpha-diolS.

    Conclusions:

    • There is no evidence for direct ovarian production of C19 conjugates.
    • Peripheral androgen action markers are influenced by ovarian androgen precursors, primarily androstenedione and testosterone.
    • Ovarian androgens serve as substrates for peripheral C19 conjugate production.