Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dugbe Nairovirus M RNA: nucleotide sequence and coding strategy.

A C Marriott1, A A el-Ghorr, P A Nuttall

  • 1NERC Institute of Virology and Environmental Microbiology, Oxford, United Kingdom.

Virology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acquired immune responses to the seasonal trivalent influenza vaccination in COPD.

Clinical and experimental immunology·2019
Same author

Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza.

BMC infectious diseases·2017
Same author

Antiplatelet-derived growth factor (PDGF) activity in the saliva of ixodid ticks is linked with their long mouthparts.

Parasite immunology·2013
Same author

Evasin-3-like anti-chemokine activity in salivary gland extracts of ixodid ticks during blood-feeding: a new target for tick control.

Parasite immunology·2010
Same author

Defective interfering viruses and their potential as antiviral agents.

Reviews in medical virology·2009
Same author

Immunomodulatory arsenal of nymphal ticks.

Medical and veterinary entomology·2008

Researchers identified the Dugbe virus M RNA segment

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • The Dugbe virus (DUG) is a Nairovirus within the Bunyaviridae family.
  • Understanding the coding capacity of its medium (M) RNA segment is crucial for viral research.

Purpose of the Study:

  • To determine the coding assignments of the DUG virus M RNA segment.
  • To identify viral proteins and their functions.

Main Methods:

  • Complete nucleotide sequencing of the DUG virus M RNA segment.
  • Sequence homology comparisons with other Bunyaviridae.
  • Expression of M RNA open reading frame fragments in Escherichia coli.
  • Amino-terminal sequencing of viral glycoproteins.

Main Results:

Related Experiment Videos

  • A single long open reading frame (4888 nucleotides) was identified in the viral complementary RNA.
  • The carboxyl-terminal region encodes the G1 structural glycoprotein, a target for neutralizing antibodies.
  • Two nonstructural glycoproteins, one processing into G1, were identified.
  • The G2 coding region is located upstream of G1.
  • A 70 kDa nonstructural protein precursor to G2 was identified.

Conclusions:

  • The DUG virus M RNA segment encodes structural glycoproteins G1 and G2, and nonstructural proteins.
  • The G1 glycoprotein is a key target for neutralizing antibodies.
  • The identified nonstructural proteins play roles in viral replication and assembly.