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Bioluminescence enhanced enzyme immunoassay for human alpha-atrial natriuretic peptide using luciferin-luciferase
M Fraysse1, F X Galen, G Habrioux
1Laboratoire de Physiologie, UFR de Pharmacie, Limoges, France.
Abstract:
A sensitive bioluminescent enzyme immunoassay (BEIA) for the determination of human alpha-atrial natriuretic peptide was developed. The usable ranges of the standard curve extend from 10 pg to 400 pg for the one-step method and from 2.5 to 50 pg for the two-step method.