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UV absorption complicates PCR decontamination
R Frothingham1, R B Blitchington, D H Lee
1Duke University Medical Center, Durham, NC 27710.
Biotechniques
|August 1, 1992
Abstract:
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.