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Eukaryotic cells grown on microcarrier beads offer a cost-efficient way to propagate Chlamydia trachomatis
J E Tam1, S T Knight, C H Davis
1Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Biotechniques
|September 1, 1992
Abstract:
The use of microcarrier cell culture as a method for the in vitro propagation of the obligate intracellular bacterial parasite, Chlamydia trachomatis, is described. The microcarrier beads proved to be a more cost-effective means to propagate C. trachomatis than traditional tissue culture flasks or roller bottles without sacrificing yields or infectivity. In addition, microcarrier cell culture was found to be a much simpler technique to study the intracellular development of these bacteria.