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Related Experiment Videos

Physiological differences among isolates of Phytophthora cinnamomi.

W D Kelley

    Canadian Journal of Microbiology
    |October 1, 1975
    PubMed
    Summary

    Four Phytophthora cinnamomi isolates showed significant differences in enzyme activities, including amylase, beta-glucosidase, and phosphatase. These variations can be measured quantitatively, offering insights into isolate-specific behaviors.

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    Interactions of Phytophthora cinnamomi and Trichoderma spp. in relation to propagule production in soil cultures at 26 degrees C1.

    Canadian journal of microbiology·1977

    Area of Science:

    • Plant Pathology
    • Soil Microbiology
    • Enzymology

    Background:

    • Phytophthora cinnamomi is a destructive plant pathogen.
    • Understanding enzyme activity variations among isolates is crucial for disease management.
    • Soil enzyme activities are key indicators of microbial function.

    Purpose of the Study:

    • To investigate and quantify differences in amylase, beta-glucosidase, and phosphatase activities among four Phytophthora cinnamomi isolates.
    • To determine the pH optima for these enzymes in different isolates.
    • To compare the relative activities of these enzymes across isolates.

    Main Methods:

    • Culturing four Phytophthora cinnamomi isolates in sterilized soil for 20 days.
    • Measuring amylase, beta-glucosidase, and phosphatase activities at various pH levels.
    • Conducting timed experiments to assess enzyme activity rates.

    Main Results:

    • Significant differences in enzyme activities were observed among the four isolates.
    • Amylase pH optima were generally around pH 5.5 for all isolates.
    • Beta-glucosidase and phosphatase activities showed distinct pH optima and varying levels across isolates, with isolate A-7725 exhibiting lower optima for these enzymes.

    Conclusions:

    • Distinct enzymatic profiles exist among Phytophthora cinnamomi isolates.
    • These quantitative differences in enzyme activity can be used to differentiate isolates.
    • The findings provide a basis for understanding isolate-specific interactions and developing targeted control strategies.

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