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Developmental changes in hepatic basolateral membrane lipid composition and fluidity.

D Novak1, W Ray, J Carver

  • 1Department of Pediatrics, University of South Florida College of Medicine, Tampa.

Biochimica Et Biophysica Acta
|October 19, 1992
PubMed
Summary
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Neonatal rat liver membranes are less fluid than adult membranes, likely due to higher cholesterol content. This impacts hepatic ion transport and membrane protein function.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Membrane fluidity and lipid composition are critical for membrane protein function.
  • Neonatal hepatic ion clearance rates are reduced compared to adults.

Purpose of the Study:

  • To investigate if hepatic basolateral membranes (HBLMs) from suckling rats exhibit reduced fluidity compared to adult rats.
  • To determine the lipid composition differences contributing to potential fluidity variations.

Main Methods:

  • Preparation of suckling (SBLMV) and adult (ABLMV) rat liver basolateral membrane vesicles using a Percoll gradient.
  • Assay of Na+/K(+)-ATPase activity.
  • Analysis of lipid composition, including cholesterol and phospholipid content.
  • Measurement of membrane fluidity using fluorescence anisotropy with diphenylhexatriene and anthroyloxy-stearate probes.

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Main Results:

  • Na+/K(+)-ATPase activity was comparable between SBLMV and ABLMV.
  • SBLMV showed significantly higher double bond index, cholesterol, and cholesterol/phosphorus ratios than ABLMV.
  • Fluorescence anisotropy indicated significantly lower fluidity in SBLMV compared to ABLMV.

Conclusions:

  • Hepatic basolateral membranes from suckling rats are less fluid than those from adult rats.
  • Increased cholesterol content in suckling rat HBLMs is a likely contributor to reduced membrane fluidity.
  • These findings may explain age-related differences in hepatic ion transport and membrane protein activity.