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Platelet heterogeneity based on lactic dehydrogenase activity.

P De Sole¹, B Zappacosta

¹Istituto di Fisiologia Umana, Servizio di Chimica Clinica, Catholic University, Rome, Italy.

Annals of Hematology

|September 1, 1992

Summary

Human platelet lactic dehydrogenase (LDH) activity varies with size. Smaller platelets show higher LDH levels, suggesting LDH can indicate in vivo platelet activation.

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Area of Science:

Hematology
Biochemistry
Cell Biology

Background:

Lactic dehydrogenase (LDH) is an enzyme found in human platelets.
Platelet subpopulations can be isolated based on size and density.
Understanding enzyme activity in different platelet populations is crucial for hematological research.

Purpose of the Study:

To investigate the relationship between platelet size and lactic dehydrogenase (LDH) activity.
To determine if LDH activity can serve as a marker for platelet activation in vivo.

Main Methods:

Human platelets were separated into subpopulations using Percoll discontinuous gradient centrifugation.
Lactic dehydrogenase (LDH) activity was measured in different platelet subpopulations.
Mean platelet volume (MPV) was correlated with LDH content.

Main Results:

A clear change in LDH activity was observed in platelets with a mean platelet volume (MPV) around 6 fl.
Platelets with MPV > 6 fl had constant LDH content (0.5 nU/fl).
Smaller platelets (3 < MPV < 6 fl) exhibited an inverse correlation between LDH content and MPV, reaching 1.5-2.0 nU/fl.

Conclusions:

Platelet LDH activity is inversely correlated with platelet size in smaller subpopulations.
Small, granule-depleted platelets show significantly higher LDH activity.
Platelet LDH activity is proposed as a potential in vivo activation index.

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