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Large-scale blood substitute production using a microfluidizer.

A Vivier1, J C Vuillemard, H W Ackermann

  • 1INRS-Santé, Pointe-Claire, Canada.

Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology
|January 1, 1992
PubMed
Summary
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Microfluidization successfully created stable liposomes for artificial blood, improving lipid and hemoglobin encapsulation. Further optimization is needed for large-scale artificial blood production using less lipid.

Area of Science:

  • Biomaterials Science
  • Chemical Engineering
  • Hematology

Background:

  • Artificial blood development requires efficient methods for encapsulating hemoglobin within liposomes.
  • Phospholipid dispersion is a critical step in creating stable liposomes for biomedical applications.

Purpose of the Study:

  • To evaluate microfluidization as a method for dispersing phospholipids in hemoglobin solutions.
  • To improve lipid incorporation and hemoglobin encapsulation efficiency in liposomes for potential artificial blood applications.

Main Methods:

  • Microfluidization was employed to disperse phospholipids in aqueous hemoglobin solutions.
  • Liposome formation and characterization (size, stability) were assessed.
  • Lipid incorporation and hemoglobin encapsulation efficiencies were quantified.

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Main Results:

  • Spherical and stable liposomes, 2-3 microns in diameter, were successfully produced.
  • Lipid incorporation reached up to 85%, and hemoglobin encapsulation up to 15%.
  • Current hemoglobin concentration in liposomes is limited to 150 g/l.

Conclusions:

  • Microfluidization is a promising technique for liposome production for artificial blood.
  • Enhanced lipid and hemoglobin incorporation were achieved compared to previous methods.
  • Further development of dispersion systems is necessary for economically viable, large-scale artificial blood production.