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Effects of X-linked hemoglobin on in-vitro platelet function
R F Reiss1, R Caballero, J Hess
1Blood Research Division, Letterman Army Institute of Research, Presidio of San Francisco, CA.
Summary
Alpha-alpha cross-linked hemoglobin did not affect platelet function in vitro. Whole blood aggregometry showed no significant differences when platelets were exposed to this hemoglobin solution compared to saline.
Area of Science:
- Biochemistry
- Hematology
- Biomaterials
Background:
- Platelet function is critical for hemostasis.
- Hemoglobin-based oxygen carriers (HBOCs) are investigated as blood substitutes.
- Assessing the impact of HBOCs on platelet function is crucial for safety.
Purpose of the Study:
- To evaluate the in vitro effect of alpha-alpha cross-linked hemoglobin on platelet aggregation.
- To compare platelet function in whole blood diluted with hemoglobin solution versus saline.
Main Methods:
- Whole blood aggregometry was performed using ADP, collagen, and ristocetin as agonists.
- Platelet aggregation was quantified by measuring impedance changes.
- Blood samples were incubated in either a 10% alpha-alpha cross-linked hemoglobin solution or saline.
Main Results:
- No significant difference in mean impedance change was observed between hemoglobin-treated and saline-treated groups.
- Spontaneous platelet aggregation did not occur during incubation without agonists.
- Alpha-alpha cross-linked hemoglobin did not impair platelet aggregation response to common agonists.
Conclusions:
- The studied concentration of alpha-alpha cross-linked hemoglobin does not adversely affect in vitro platelet function.
- This finding supports the potential use of this HBOC in applications where platelet function is critical.