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Related Experiment Videos

Biocompatibility studies with perfluorochemical oxygen carriers.

K C Lowe1, F H Armstrong

  • 1Department of Life Science, University of Nottingham, U.K.

Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology
|January 1, 1992
PubMed
Summary

Novel perfluorodecalin (FDC) emulsions impact liver enzymes in rats, increasing cytochrome P-450 and aryl esterase. These perfluorochemicals may alter drug metabolism, as indicated by reduced anesthetic sleeping times.

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Area of Science:

  • Biochemistry
  • Toxicology
  • Pharmacology

Background:

  • Perfluorochemicals (PFCs) are synthetic compounds with unique properties.
  • Their impact on liver metabolic enzymes requires further investigation.
  • Understanding these effects is crucial for assessing potential health implications.

Purpose of the Study:

  • To investigate the effects of a novel perfluorodecalin (FDC)-based emulsion and other PFCs on rat liver enzymes.
  • To determine the influence of these substances on cytochrome P-450 (P-450) and liver aryl esterase (LAE) activity.
  • To assess the potential impact on drug metabolism, using anesthetic sleeping time as an indicator.

Main Methods:

  • Male rats were injected with either a novel FDC emulsion, specific PFC oils, or saline.

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  • Liver P-450 concentration and LAE activity were measured.
  • Pentobarbital-induced sleeping time was recorded after novel emulsion or saline pretreatment.

    • Main Results:

    • Injection of the novel emulsion or FDC significantly increased mean P-450 concentration.
    • LAE activity increased following FDC and perfluoroperhydrofluoranthrene (a C-16 oil) administration.
    • No significant changes in P-450 or LAE were observed with other tested PFCs.
    • Rats pretreated with the novel emulsion exhibited reduced anesthetic sleeping times compared to controls.

    Conclusions:

    • The novel FDC emulsion and certain PFCs can modulate key liver metabolic enzymes in rats.
    • These alterations in P-450 and LAE suggest potential impacts on xenobiotic metabolism.
    • The observed decrease in sleeping time indicates that PFCs may affect drug pharmacokinetics.