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Related Experiment Videos

Factor Xa interacts with two sites on monocytes with different functional activities.

L A Worfolk1, R A Robinson, P B Tracy

  • 1Department of Cell and Molecular Biology, University of Vermont College of Medicine, Burlington 05405.

Blood
|October 15, 1992
PubMed
Summary

Monocytes bind factor Xa (FXa) via two distinct membrane sites. Factor Va (FVa) presence dictates FXa

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Area of Science:

  • Biochemistry
  • Hematology
  • Cell Biology

Background:

  • Factor Xa (FXa) plays a critical role in the coagulation cascade, primarily in prothrombin activation.
  • Monocytes present binding sites that can influence coagulation factor activity.
  • The role of factor Va (FVa) in modulating FXa interactions on cell surfaces is not fully understood.

Purpose of the Study:

  • To investigate the functional significance of factor Xa (FXa) interactions at the monocyte membrane.
  • To elucidate the role of factor Va (FVa) in modulating FXa substrate specificity on monocytes.
  • To differentiate the mechanisms of FXa action in the presence and absence of FVa.

Main Methods:

  • Studied FXa-catalyzed prothrombin and factor IX cleavage on monocyte surfaces.

Related Experiment Videos

  • Investigated the effect of added factor Va (FVa) on these reactions.
  • Utilized a monoclonal antibody against FVa light chain (alpha-HFV1) for inhibition studies.
  • Compared FXa activity on monocytes with activity on phospholipid vesicles and platelets.

    • Main Results:

    • Prothrombin activation by FXa on monocytes required FVa, forming a membrane-bound complex.
    • FXa alone on monocytes cleaved factor IX, a reaction dependent on monocyte and FXa concentration.
    • FVa modulated FXa substrate specificity on monocytes but not on phospholipid vesicles.
    • Monocyte-bound FXa interactions were distinguishable by FVa requirement and antibody inhibition profiles.

    Conclusions:

    • Monocytes provide distinct membrane sites that modulate FXa binding and activity.
    • Factor Va (FVa) availability dictates whether FXa activates prothrombin or cleaves factor IX on monocytes.
    • These findings highlight the monocyte's role in regulating coagulation factor specificity.