Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Blood-brain Barrier

The Blood-brain Barrier

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...

Cerebral Edema ll: Pathophysiology

Cerebral Edema ll: Pathophysiology

Vasogenic edema is a major form of cerebral edema characterized by abnormal accumulation of fluid in the brain’s extracellular space due to disruption of the blood–brain barrier (BBB). The BBB is a specialized structure composed of endothelial cells connected by tight junctions, supported by astrocytic endfeet and a basement membrane. Under normal conditions, it tightly regulates the movement of ions, proteins, and solutes between the bloodstream and brain parenchyma. When this barrier loses...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Progress in research and application of respiratory surface electromyography in respiratory and critical care medicine].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024

Same author

[Primary ciliary dyskinesia caused by mutation of CCNO29 gene in a family report].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2024

Same author

[Effect of sugammadex on postoperative nausea and vomiting after surgery for intracranial aneurysm].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2023

Same author

[Construction and analysis of functional network of hemi-brain in patients with brain tumors before and after anesthesia based on resting-state functional magnetic resonance imaging].

Zhonghua yi xue za zhi·2023

Same author

[Effectiveness and safety of ultrasound-guided microwave ablation for the treatment of primary hyperparathyroidism in 12 patients with parathyroid adenoma].

Zhonghua nei ke za zhi·2021

Same author

[A dose-response meta-analysis on the relationship between daily tea intake and cardiovascular mortality based on the GRADE system].

Zhonghua xin xue guan bing za zhi·2021

Search research articles

Related Experiment Video

Updated: Jul 28, 2026

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Published on: September 12, 2018

Capillary NMDA receptors regulate blood-brain barrier function and breakdown.

H Koenig¹, J J Trout, A D Goldstone

¹Neurology Service, VA Lakeside Medical Center, Chicago IL 60611.

|August 21, 1992

Summary

NMDA receptors and polyamines contribute to blood-brain barrier (BBB) breakdown after injury. Blocking NMDA receptors with MK-801 prevents BBB disruption, suggesting a key role for these receptors in BBB regulation.

More Related Videos

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

Published on: May 7, 2018

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Related Experiment Videos

Last Updated: Jul 28, 2026

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Published on: September 12, 2018

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

Published on: May 7, 2018

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Area of Science:

Neuroscience
Biochemistry
Physiology

Background:

Polyamines and ornithine decarboxylase (ODC) are linked to blood-brain barrier (BBB) disruption after cryogenic injury.
MK-801, an NMDA receptor antagonist, prevents ODC activation and BBB breakdown, suggesting NMDA receptor involvement.

Purpose of the Study:

To investigate the role of NMDA receptors in regulating BBB function and nutrient transport.
To explore the link between NMDA receptors, ODC, polyamines, and calcium influx in cerebral capillaries.

Main Methods:

Studies were conducted on isolated rat cerebral capillaries.
NMDA receptor activation was used to stimulate capillary uptake of horseradish peroxidase, 2-deoxy-[14C]glucose, and 45Ca.

Main Results:

NMDA stimulation increased capillary uptake in a receptor-, concentration-, polyamine-, and Ca(2+)-dependent manner.
These findings support the presence of NMDA receptors linked to ODC in cerebral capillaries.

Conclusions:

NMDA receptors may couple nutrient transport to glutamate-mediated neuronal excitation.
Overstimulation of NMDA receptors can disrupt normal blood-brain barrier function.