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Choline acetyltransferase expression studied with an oligonucleotide probe
1Department of Anatomy, University of Mississippi Medical Center, Jackson 39216-4505.
Cellular and Molecular Neurobiology
|August 1, 1992
Abstract:
1. The localization of choline acetyltransferase messenger RNA has been studied using a digoxigenin-tailed complementary oligodeoxynucleotide probe for in situ hybridization. 2. Putative cholinergic cells of the rat and ferret spinal cord and the ferret retina were labeled. 3. This technique affords superior resolution compared to radioactively labeled probes, with apparently equal sensitivity.