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Updated: Aug 17, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
Published on: June 23, 2022
Ballistic disruption of Penicillium chrysogenum cells
Abstract:
The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans. Some of the factors influencing breakage are discussed.
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