Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro dissolution of curium oxide using a phagolysosomal simulant solvent system.

S Y Helfinstine1, R A Guilmette, G A Schlapper

  • 1Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185.

Environmental Health Perspectives
|July 1, 1992
PubMed
Summary

Researchers developed a phagolysosomal simulant solvent (PSS) to study actinide oxide dissolution in vitro. This new system overcomes limitations of short-lived alveolar macrophages (AM) for long-term dissolution studies of actinides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A BRIEF OVERVIEW OF COMPARTMENTAL MODELING FOR INTAKE OF PLUTONIUM VIA WOUNDS.

Radiation protection dosimetry·2017
Same author

Internal dosimetry verification and validation database.

Radiation protection dosimetry·2008
Same author

Updating the ICRP human respiratory tract model.

Radiation protection dosimetry·2008
Same author

Comparison of dose estimation from occupational exposure to 239Pu using different modelling approaches.

Radiation protection dosimetry·2007
Same author

Internal dose assessment data management system for a large population of Pu workers.

Radiation protection dosimetry·2007
Same author

In vitro dissolution study of plutonium in aerosol particles from the Mayak PA: a tool for individualised dose estimates.

Radiation protection dosimetry·2007

Area of Science:

  • Environmental Science
  • Toxicology
  • Radiochemistry

Background:

  • Studying actinide oxide behavior in alveolar macrophages (AM) in vitro is challenging due to the limited lifespan of these cells.
  • A need exists for a stable in vitro system to accurately assess the dissolution of poorly soluble actinide materials.

Purpose of the Study:

  • To develop and validate an in vitro dissolution system, the phagolysosomal simulant solvent (PSS), that mimics the acidic intracellular environment.
  • To compare actinide dissolution in the PSS with dissolution in cultured canine AM to optimize the PSS for future studies.

Main Methods:

  • Created a phagolysosomal simulant solvent (PSS) using varying concentrations of HCl and diethylenetriamine pentaacetate (DTPA) in distilled water.
  • Tested the PSS with curium sequioxide (244Cm2O3) at different pH levels (4.0-6.0) and DTPA-to-curium ratios (1000:1, 100:1, 10:1).

Related Experiment Videos

  • Collected and analyzed samples daily for 7 days using ultracentrifugation and filtration to quantify solubilized curium.

    • Main Results:

    • The PSS system allowed for indefinite maintenance, overcoming the limitations of short-lived alveolar macrophages.
    • Dissolution of 244Cm2O3 was measurable within the PSS over the 7-day study period.
    • Data was generated to compare PSS dissolution with dissolution occurring within cultured AM.

    Conclusions:

    • The developed phagolysosomal simulant solvent (PSS) provides a viable and stable in vitro model for studying actinide oxide dissolution.
    • This PSS system is suitable for long-term investigations of poorly soluble actinide materials, offering an alternative to traditional cell culture methods.