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In vitro dissolution of curium oxide using a phagolysosomal simulant solvent system
S Y Helfinstine1, R A Guilmette, G A Schlapper
1Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185.
Environmental Health Perspectives
|July 1, 1992
Summary
Researchers developed a phagolysosomal simulant solvent (PSS) to study actinide oxide dissolution in vitro. This new system overcomes limitations of short-lived alveolar macrophages (AM) for long-term dissolution studies of actinides.
Area of Science:
- Environmental Science
- Toxicology
- Radiochemistry
Background:
- Studying actinide oxide behavior in alveolar macrophages (AM) in vitro is challenging due to the limited lifespan of these cells.
- A need exists for a stable in vitro system to accurately assess the dissolution of poorly soluble actinide materials.
Purpose of the Study:
- To develop and validate an in vitro dissolution system, the phagolysosomal simulant solvent (PSS), that mimics the acidic intracellular environment.
- To compare actinide dissolution in the PSS with dissolution in cultured canine AM to optimize the PSS for future studies.
Main Methods:
- Created a phagolysosomal simulant solvent (PSS) using varying concentrations of HCl and diethylenetriamine pentaacetate (DTPA) in distilled water.
- Tested the PSS with curium sequioxide (244Cm2O3) at different pH levels (4.0-6.0) and DTPA-to-curium ratios (1000:1, 100:1, 10:1).
- Collected and analyzed samples daily for 7 days using ultracentrifugation and filtration to quantify solubilized curium.
Main Results:
- The PSS system allowed for indefinite maintenance, overcoming the limitations of short-lived alveolar macrophages.
- Dissolution of 244Cm2O3 was measurable within the PSS over the 7-day study period.
- Data was generated to compare PSS dissolution with dissolution occurring within cultured AM.
Conclusions:
- The developed phagolysosomal simulant solvent (PSS) provides a viable and stable in vitro model for studying actinide oxide dissolution.
- This PSS system is suitable for long-term investigations of poorly soluble actinide materials, offering an alternative to traditional cell culture methods.