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The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation
D Y Mason1, C J van Noesel, J L Cordell
1Department of Haematology, John Radcliffe Hospital, Headington, Oxford, GB.
European Journal of Immunology
|October 1, 1992
Summary
The B29 gene product is part of the human B cell heterodimer, but its expression differs from mb-1 during B cell development and in acute lymphoblastic leukemia.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Surface immunoglobulin on B cells is associated with a heterodimer of mb-1 and B29 gene products.
- Understanding the differential expression of these components is crucial for B cell development research.
Purpose of the Study:
- To confirm if the human B cell heterodimer's 37-kDa component is encoded by the B29 gene.
- To compare the expression patterns of B29 and mb-1 in human B cell development and acute lymphoblastic leukemia (ALL).
Main Methods:
- Antibodies were raised against a peptide sequence from the intracytoplasmic C terminus of the B29 murine gene product.
- Immunocytochemical reactivity of anti-B29 and anti-mb-1 antibodies was assessed in ALL cases and lymphoid tissue sections.
Main Results:
- Anti-B29 antibodies detected the 37-kDa component of the human heterodimer, confirming B29 gene involvement.
- mb-1 was expressed in 24/25 ALL cases, while B29 was expressed in only 13/25, often in pre-B ALL cases.
- Anti-B29 labeled B cell follicles but not plasma cells, whereas anti-mb-1 labeled both, indicating differential expression timing.
Conclusions:
- B29 gene encodes a component of the human B cell heterodimer.
- B29 synthesis initiates later than mb-1 during B cell precursor development and ceases before the plasma cell stage.