Related Experiment Videos
Conformation and length of the signal sequence affect processing of secretory protein
A Kohara1, Y Yamamoto, M Kikuchi
1Protein Engineering Research Institute, Osaka, Japan.
FEBS Letters
|October 26, 1992
Abstract:
Processing of human lysozyme with artificially designed signal sequences was examined in an in vitro translation-translocation system and compared with their secretory capabilities in yeast. It has been shown that the conformation of the C-terminal region of the signal sequence and the length of the hydrophobic segment are important factors for efficient cleavage of the signal sequence.