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Liposome mediated affection of monocytes
I Huitinga1, J G Damoiseaux, N van Rooijen
1Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Immunobiology
|June 1, 1992
Summary
Liposomes containing dichloromethylene diphosphonate (Cl2MDP) eliminate circulating macrophages. This study found Cl2MDP liposomes affect rat monocytes but not bone marrow macrophage precursors.
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Background:
- Dichloromethylene diphosphonate (Cl2MDP) encapsulated in liposomes targets phagocytic macrophages in the spleen and liver.
- Liposomes, particularly mannosylated ones (PCMAN), enhance drug delivery to macrophages.
Purpose of the Study:
- To investigate the effects of Cl2MDP-containing liposomes on rat monocytes and bone marrow macrophage precursors (BMMP's).
- To compare the efficacy of standard Phosphatidylcholine-Cholesterol (PC) liposomes versus PCMAN liposomes in targeting these cells.
Main Methods:
- Intravenous injection of Cl2MDP-loaded PC and PCMAN liposomes into rats.
- Isolation of blood leukocytes and bone marrow cells at 24 hours and one week post-injection.
- Culturing of isolated cells to assess macrophage presence and differentiation.
Main Results:
- Cl2MDP liposomes (both PC and PCMAN) significantly reduced the number of monocytes in rats 24 hours after injection.
- Macrophage populations derived from blood leukocytes of treated rats were nearly absent in cultures.
- Bone marrow macrophage precursors (BMMP's) remained unaffected by the Cl2MDP liposome treatment.
Conclusions:
- Cl2MDP-loaded liposomes effectively deplete circulating monocytes and their macrophage progeny.
- PCMAN liposomes show similar efficacy to PC liposomes in affecting monocytes.
- The study highlights the potential of Cl2MDP liposomes for modulating monocyte/macrophage populations, sparing bone marrow precursors.