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Related Experiment Videos

Invasin expression in Yersinia pseudotuberculosis.

M Simonet1, S Falkow

  • 1Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades, Paris, France.

Infection and Immunity
|October 1, 1992
PubMed
Summary
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Yersinia pseudotuberculosis invasin protein mediates bacterial entry into host cells. Higher invasin levels correlate with increased cell invasion and spleen colonization in mice, indicating its role in virulence.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Bacterial genetics

Background:

  • Yersinia pseudotuberculosis is an important human and animal pathogen.
  • The bacterial outer membrane protein invasin mediates entry into host cells.
  • The inv gene encodes invasin.

Purpose of the Study:

  • To investigate the presence and expression of invasin in Yersinia pseudotuberculosis strains.
  • To correlate invasin levels with bacterial invasiveness and virulence in animal models.

Main Methods:

  • Analysis of inv-related sequences using a radiolabeled inv clone (pRI203).
  • Western immunoblot analysis of invasin production using monoclonal antibodies.
  • Assessment of bacterial entry into cultured HEp-2 and CHO cells.

Related Experiment Videos

  • Murine models to evaluate virulence (spleen colonization, mesenteric lymph node translocation).

    • Main Results:

    • All Y. pseudotuberculosis strains analyzed possessed inv-specific sequences.
    • Most strains produced invasin, with varying levels observed.
    • Invasin production strongly correlated with bacterial entry into cultured cells.
    • Bacterial cell invasion and spleen colonization in mice were highly correlated.
    • Intestinal translocation to mesenteric lymph nodes did not correlate with in vitro invasiveness.

    Conclusions:

    • Invasin expression is a key factor in Yersinia pseudotuberculosis virulence.
    • Invasin facilitates bacterial invasion of host cells and systemic spread (spleen colonization).
    • In vitro invasiveness is a reliable predictor of spleen colonization but not intestinal translocation.