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Is there a plasma membrane-located anion-sensitive ATPase?

J M van Amelsvoort, J J de Pont, S L Bonting

Biochimica Et Biophysica Acta

|April 18, 1977

Summary

This study investigated the location of a specific enzyme in trout gills, rabbit kidneys, and gastric mucosa. Findings suggest the enzyme is primarily located in mitochondria within trout gills and rabbit gastric mucosa.

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Area of Science:

Biochemistry
Cell Biology
Physiology

Background:

Magnesium-dependent ATPase activity is crucial for cellular functions.
Previous studies on the localization of HCO3-stimulated ATPase have yielded conflicting results.
Understanding enzyme localization is key to elucidating cellular transport mechanisms.

Purpose of the Study:

To determine the intracellular localization of a specific magnesium-dependent ATPase.
To investigate the enzyme's presence in rainbow trout gill, rabbit kidney, and rabbit gastric mucosa.
To clarify discrepancies in previous enzyme localization studies.

Main Methods:

Tissue homogenization and centrifugal fractionation.
Density gradient centrifugation of microsomal and light mitochondrial fractions.
Characterization using marker enzyme assays and electron microscopy.

Main Results:

Trout gill tissue showed strong indications of exclusively mitochondrial localization for the enzyme.
Rabbit gastric mucosa initially suggested a non-mitochondrial enzyme, but further analysis pointed to mitochondrial inner membrane localization.
No definitive localization could be concluded for rabbit kidney tissue.

Conclusions:

The HCO3-stimulated, SCN--inhibited magnesium-dependent ATPase is predominantly mitochondrial in trout gill and rabbit gastric mucosa.
The enzyme in gastric mucosa likely resides within the mitochondrial inner membranes.
Further research may resolve conflicting data and clarify the enzyme's role in different tissues.

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