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Tryptophan biosynthesis genes in Lactococcus lactis subsp. lactis.

J Bardowski1, S D Ehrlich, A Chopin

  • 1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

Journal of Bacteriology
|October 1, 1992
PubMed
Summary

Researchers characterized the tryptophan biosynthesis genes in Lactococcus lactis, identifying seven genes in the order trpEGDCFBA. Gene regulation likely differs from E. coli, possibly resembling Bacillus spp. mechanisms.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Tryptophan biosynthesis is essential for bacterial growth.
  • Understanding gene organization and regulation provides insights into metabolic pathways.

Purpose of the Study:

  • To characterize the chromosomal region encoding tryptophan biosynthesis genes in Lactococcus lactis.
  • To elucidate the gene order, structure, and potential regulatory mechanisms of these genes.

Main Methods:

  • Gene cloning and DNA sequencing.
  • Homology analysis of gene products.
  • Complementation of Escherichia coli tryptophan auxotrophic mutations.

Main Results:

  • Identified seven structural genes for tryptophan biosynthesis (trpEGDCFBA) spanning 7,968 bp.

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  • Confirmed gene identification through homology and functional complementation.
  • Observed overlapping gene pairs and specific intercistronic spacing.
  • Identified potential promoter and terminator sequences upstream of the gene cluster.
  • Ruled out attenuation-based regulation similar to E. coli due to lack of a leader peptide.

    • Conclusions:

    • The organization of tryptophan biosynthesis genes in L. lactis is distinct.
    • Regulation of tryptophan biosynthesis in L. lactis may be analogous to mechanisms found in Bacillus species.
    • Further investigation into L. lactis trp gene regulation is warranted.