Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histidine biosynthesis genes in Lactococcus lactis subsp. lactis.

C Delorme1, S D Ehrlich, P Renault

  • 1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

Journal of Bacteriology
|October 1, 1992
PubMed
Summary

Researchers identified Lactococcus lactis genes for histidine biosynthesis using genetic complementation and DNA sequencing. They discovered a potential operon with genes for histidine synthesis and other unique proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lebanese fermented goat milk products: From tradition to meta-omics.

Food research international (Ottawa, Ont.)·2023
Same author

Olfactory and gustatory dysfunctions in COVID-19 outpatients: A prospective cohort study.

Infectious diseases now·2021
Same author

Efficient transformation of Lactobacillus sake by electroporation.

Microbiology (Reading, England)·2021
Same author

COVID-19-related encephalopathy: a case series with brain FDG-positron-emission tomography/computed tomography findings.

European journal of neurology·2020
Same author

Multimodal neurometabolic investigation of the effects of zolpidem on leukoencephalopathy-related apathy.

European journal of neurology·2020
Same author

Management of neuropathic pain induced by surgery: Review of the literature by a group of experts specialized in pain management, anesthesia and surgery.

Journal of visceral surgery·2019

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Lactococcus lactis is a key bacterium in dairy fermentation.
  • Understanding its metabolic pathways, like histidine biosynthesis, is crucial for strain improvement.
  • Genetic tools for Lactococcus lactis are essential for functional analysis.

Purpose of the Study:

  • To clone and characterize genes involved in histidine biosynthesis in Lactococcus lactis subsp. lactis.

Main Methods:

  • Gene cloning and characterization through complementation of Escherichia coli and Bacillus subtilis mutants.
  • DNA sequencing of the Lactococcus lactis region containing histidine biosynthesis genes.
  • Bioinformatic analysis of open reading frames (ORFs) and predicted protein homology.

Related Experiment Videos

Main Results:

  • Identified and localized Lactococcus lactis genes homologous to known histidine biosynthesis enzymes.
  • Discovered a 11.5-kb DNA region containing 14 ORFs, potentially forming an operon.
  • Found ORFs encoding proteins similar to histidyl-tRNA synthetases (with altered function) and 3'-aminoglycoside phosphotransferases (without antibiotic resistance).

Conclusions:

  • The identified genes and operon provide insights into histidine biosynthesis regulation in Lactococcus lactis.
  • Novel ORFs suggest unique roles for these genes beyond canonical histidine synthesis.
  • This study lays the groundwork for genetic manipulation of Lactococcus lactis for enhanced traits.