Related Experiment Videos
Direct serum injection in ion chromatography on packing materials with a semi-permeable surface
W Buchberger1, H Malissa, E Mülleder
1Department of Analytical Chemistry, Johannes-Kepler-University, Linz, Austria.
Journal of Chromatography
|June 5, 1992
Abstract:
Reversed-phase packing materials with restricted access of proteins to the hydrophobic sites were tested for their applicability in the ion-interaction chromatography of anions. Especially C8-modified silica, which had been coated with a hydrophilic polymer acting as a semi-permeable barrier, could be used successfully for the separation of several anions in a proteinaceous matrix without removing the proteins prior to injection. In combination with UV or conductivity detection, this technique allows the determination of some physiologically important anions in serum samples.