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Updated: Aug 11, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
Human tonsil intraepithelial B cells: a marginal zone-related subpopulation
M Morente1, M A Piris, J L Orradre
1Department of Pathology, Hospital General de Guadalajara, Universidad de Alcalá de Henares, Spain.
Intraepithelial B cells in human tonsils share characteristics with splenic marginal zone cells. These findings suggest a potential role in mucosa-associated lymphoid tissue (MALT) lymphomas.
Area of Science:
- Immunology
- Cell Biology
- Histopathology
Background:
- The marginal zone (MZ) of secondary lymphoid organs plays a crucial role in immune responses.
- Understanding B cell populations in human tonsils is important for insights into mucosal immunity.
Purpose of the Study:
- To investigate the characteristics of intraepithelial B cells in human palatine tonsils.
- To compare these cells with marginal zone B cells in spleen and Peyer's patches.
Main Methods:
- Analysis of reactive human palatine tonsils using light and electron microscopy.
- Immunophenotyping of intraepithelial B cells with monoclonal antibodies.
Main Results:
- Abundant intraepithelial B cells with centrocyte-like morphology were found in the tonsillar cryptal epithelium.
- These cells exhibited an immunophenotype (IgM+, IgD-, CD23-, CD10-, CD35+, CD21+) similar to splenic marginal zone B cells.
Conclusions:
- Intraepithelial B cells in human tonsils may be analogous to marginal zone cells in Peyer's patches.
- Their presence in the epithelium could be relevant to the pathogenesis of mucosa-associated lymphoid tissue (MALT) lymphomas.
- Human tonsil lymphoid tissue shares features with MALT.
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