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Selected cryopreservatives for long term storage of Helicobacter pylori at low temperatures
M Shahamat1, C Paszko-Kolva, U E Mai
1Department of Microbiology, University of Maryland, College Park 20742.
Abstract:
To meet the need for information on cryopreservation, a study was done on 32 Helicobacter pylori strains, comparing different cryopreservative media. Sheep blood, horse blood, horse serum with and without glycerol, and mineral oil media were used for long term storage of H pylori at -70 degrees C or in liquid nitrogen. Procedures were developed which permitted recovery of 87.5% of the strains included in the study after they had been stored for 24 months. Of those strains stored for more than three years, 60% were recovered. It is concluded that most strains of H pylori can be stored for up to one year or longer, under refrigeration, at -70 degrees C or in liquid nitrogen.
Insights
Cryopreservation of Helicobacter pylori is feasible using various media for long-term storage at -70°C or in liquid nitrogen. Most strains remain viable for over a year, with significant recovery rates after extended periods.
Area of Science:
- Microbiology
- Cryobiology
Background:
- Helicobacter pylori is a significant human pathogen.
- Effective cryopreservation methods are crucial for maintaining bacterial strain viability for research.
Purpose of the Study:
- To evaluate the efficacy of different cryopreservative media for long-term storage of Helicobacter pylori.
- To determine optimal conditions for H. pylori cryopreservation.
Main Methods:
- Comparison of cryopreservation media including sheep blood, horse blood, horse serum (with and without glycerol), and mineral oil.
- Storage of 32 H. pylori strains at -70°C and in liquid nitrogen for up to three years.
Main Results:
- Successful recovery of 87.5% of H. pylori strains after 24 months of storage.
- Viability of 60% of strains was confirmed after storage exceeding three years.
Conclusions:
- Most Helicobacter pylori strains can be successfully cryopreserved for extended periods (over one year).
- Storage at -70°C or in liquid nitrogen provides a reliable method for long-term H. pylori preservation.