Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New selective medium for isolating Clostridium difficile from faeces.

S T Aspinall1, D N Hutchinson

  • 1Preston Public Health Laboratory, Royal Preston Hospital.

Journal of Clinical Pathology
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Congenital rubella: Infections in hospital staff.

British medical journal·2010
Same author

Quantum phase retrieval of a Rydberg wave packet using a half-cycle pulse.

Physical review letters·2001
Same author

Associations between heat-stable (O) and heat-labile (HL) serogroup antigens of Campylobacter jejuni: evidence for interstrain relationships within three O/HL serovars.

Journal of clinical microbiology·1998
Same author

The application of genotyping techniques to the epidemiological analysis of Campylobacter jejuni.

Epidemiology and infection·1996
Same author

A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis.

The Journal of applied bacteriology·1996
Same author

How to produce a hospital disinfection policy.

The Journal of hospital infection·1994
Same journal

Defining biochemical, pathological and molecular factors prognostic in terms of disease control and survival in high-grade extremity soft tissue sarcoma: a scoping review.

Journal of clinical pathology·2026
Same journal

MILGDF: a multi-task, instance-level supervised model for oral squamous cell carcinoma integrating local-global attention and dynamic decision fusion.

Journal of clinical pathology·2026
Same journal

Paediatric B-lymphoblastic leukaemia with low peripheral blasts: a potential diagnostic pitfall.

Journal of clinical pathology·2026
Same journal

MRI-targeted versus systematic needle core biopsies in prostate cancer: a patient-based analysis of potential diagnostic and biologic underestimation.

Journal of clinical pathology·2026
Same journal

Basal plasmacytosis and eosinophilia for distinguishing inflammatory bowel disease from gastrointestinal tuberculosis on mucosal biopsy.

Journal of clinical pathology·2026
Same journal

Assay-dependent variability in free thyroxine (FT4): differential interference related to immunoassay design in a patient with subclinical hypothyroidism.

Journal of clinical pathology·2026
See all related articles

A new selective medium, CDMN, containing cysteine hydrochloride, norfloxacin, and moxalactam, is superior for isolating Clostridium difficile. CDMN improves isolation rates and reduces contamination compared to CCFA in direct fecal cultures.

Area of Science:

  • Clinical microbiology
  • Bacterial culture techniques
  • Infectious disease diagnostics

Background:

  • Clostridium difficile is a significant cause of healthcare-associated diarrhea.
  • Accurate and efficient isolation of C. difficile from fecal samples is crucial for diagnosis and treatment.
  • Current selective media like CCFA have limitations in sensitivity and specificity.

Purpose of the Study:

  • To evaluate a novel selective medium, CDMN (cysteine hydrochloride, norfloxacin, moxalactam), for C. difficile isolation.
  • To compare the performance of CDMN against the established CCFA medium.
  • To assess the impact of CDMN on isolation rates and contamination levels.

Main Methods:

  • Determination of minimum inhibitory concentrations (MICs) for norfloxacin and moxalactam against various bacterial strains.

Related Experiment Videos

  • Preparation of CDMN medium by incorporating specific antibiotic concentrations into C. difficile agar base.
  • Direct fecal culture comparison of CDMN and CCFA using 832 patient samples.

    • Main Results:

    • Norfloxacin and moxalactam effectively inhibited common fecal contaminants like enterobacteriaceae, streptococci, and Bacteroides species.
    • The combination of antibiotics in CDMN did not affect C. difficile growth or colonial morphology.
    • CDMN isolated 20% more C. difficile strains and reduced contaminating colonies by 30% compared to CCFA.

    Conclusions:

    • CDMN medium demonstrates enhanced efficacy in isolating Clostridium difficile from fecal specimens.
    • The novel CDMN medium offers improved sensitivity and reduced contamination, outperforming CCFA.
    • CDMN represents a valuable advancement in the laboratory diagnosis of C. difficile infections.