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Plaque quantitation through protein measurement.

A Smit1, P E Cleaton-Jones, M E Boardman

  • 1University of the Witwatersrand.

The Journal of the Dental Association of South Africa = Die Tydskrif Van Die Tandheelkundige Vereniging Van Suid-Afrika
|July 1, 1992
PubMed
Summary
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Quantifying dental plaque protein using a dye-binding method (Coomassie G-250) can serve as a reliable index for plaque amount. This protein quantitation method aids in standardizing bacterial counts and estimating collected plaque volume.

Area of Science:

  • Biochemistry
  • Microbiology
  • Dental Research

Background:

  • Dental plaque quantification is crucial for understanding oral health.
  • Accurate measurement of plaque biomass is essential for research and clinical assessment.

Purpose of the Study:

  • To determine if dental plaque protein quantitation using Coomassie G-250 dye-binding assay can serve as an index for plaque amount.
  • To evaluate the reliability of protein concentration as a measure of sampled dental plaque.

Main Methods:

  • Dental plaque samples were collected from 10 sites in 34 children over 5 occasions at 4-month intervals.
  • Protein concentration in 1391 plaque samples was measured using the Coomassie G-250 dye-binding method.
  • A three-way analysis of variance was employed to analyze the data.

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Main Results:

  • The mean protein concentration across all samples was 6.9 ± 4.1 µg.
  • Plaque protein concentration showed no significant difference between sampling sites within the same child (p = 0.14).
  • Significant differences in plaque protein concentration were observed over time (p = 0.0001) and between individuals (p = 0.0001).

Conclusions:

  • Dental plaque protein concentration, measured by Coomassie G-250, can be used as an index for plaque amount.
  • This method allows for the expression of plaque bacterial counts in protein units, facilitating standardization.
  • The protein quantitation method provides a useful way to relate viable bacteria counts to the estimated plaque volume collected.