Related Experiment Videos
[Enteropathogenicity and enteropathogenic toxin production of Vibrio mimicus]
Abstract:
Enteropathogenicity and enteroreactive-toxins were examined in 66 strains of Vibrio mimicus and the following results were obtained. Frequencies of enteropathogenic strains judged by the result of suckling mouse tests were 11/13 (85%) for clinical isolates and 37/53 (70%) for fish or environmental isolates. Frequencies of preservation of cholera toxin gene and NAG-ST gene were 2 and 15%, respectively, for 48 enteropathogenic strains, and 0 and 6%, respectively, for 18 non-enteropathogenic strains. Frequencies of production of NAG-rTDH, FAF and hemolysin were 4, 63 and 100%, respectively, for 48 enteropathogenic strains, and 6, 50, and 100%, respectively, for 18 non-enteropathogenic strains. No correlation between serovar and enteropathogenicity was observed in the suckling mouse test. Six out of 12 enteropathogenic strains produced hemolysin in ligated rabbit ileal loop, while 1 out of 12 non-enteropathogenic strains did so under the same condition. A significant inhibition of fluid accumulation in the ligated rabbit ileal loop test with viable cells was noted in rabbits immunized with hemolysin of non-O1 V. cholerae. These results suggest that approximately two-thirds of environmental isolates are enteropathogenic and that hemolysin is the most important toxin in the enteropathogenic mechanism of V. mimicus strains.
Insights
Vibrio mimicus strains, particularly environmental ones, frequently cause enteropathogenicity. Hemolysin appears to be the primary toxin responsible for this mechanism in V. mimicus.
Area of Science:
- Microbiology
- Bacteriology
Context:
- Vibrio mimicus is an important bacterial species with potential public health implications.
- Understanding the virulence factors of V. mimicus is crucial for assessing its enteropathogenic potential.
Purpose:
- To investigate the enteropathogenicity of Vibrio mimicus strains.
- To identify and characterize the enteroreactive toxins produced by V. mimicus.
- To determine the role of hemolysin in the pathogenesis of V. mimicus infections.
Summary:
- Enteropathogenicity was observed in 85% of clinical and 70% of environmental/fish V. mimicus isolates.
- While cholera toxin and NAG-ST genes were rarely found, NAG-rTDH, FAF, and hemolysin were frequently produced by enteropathogenic strains.
- Hemolysin production was significantly higher in enteropathogenic strains and demonstrated a role in the enteropathogenic mechanism, as evidenced by rabbit ileal loop tests and immunization studies.
Impact:
- These findings indicate that a substantial proportion of environmental V. mimicus isolates are enteropathogenic.
- Hemolysin is identified as the key virulence factor contributing to the enteropathogenicity of V. mimicus.
- This research provides valuable insights for the diagnosis and management of V. mimicus-associated gastroenteritis.