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Related Experiment Videos

Stimulus deprivation increases pineal Gs alpha and G beta.

T Babila1, D C Klein

  • 1Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

Journal of Neurochemistry
|October 1, 1992
PubMed
Summary

Stimulus deprivation, like denervation, increases both alpha and beta subunits of the GTP-binding protein Gs (Gs alpha and G beta) in the pineal gland. This effect, known as denervation supersensitivity, is mediated by adrenergic stimulation.

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Area of Science:

  • Neuroendocrinology
  • Molecular Biology
  • Cell Signaling

Background:

  • Denervation supersensitivity describes heightened responses after stimulus deprivation.
  • The cyclic AMP response to norepinephrine in the pineal gland is a known example.
  • The impact of stimulus deprivation on Gs protein subunits was previously unclear.

Purpose of the Study:

  • To investigate if stimulus deprivation alters alpha and beta subunits of the GTP binding regulatory protein Gs (Gs alpha and G beta).

Main Methods:

  • Stimulus deprivation induced via denervation, decentralization, or constant light exposure.
  • Quantification of Gs alpha and G beta using semiquantitative western blot.
  • Cholera toxin-dependent ADP-ribosylation assays for Gs alpha.
  • In vitro organ culture studies.

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  • Pharmacological intervention with isoproterenol.

    • Main Results:

    • Denervation, decentralization, and constant light exposure increased Gs alpha and G beta subunits up to fourfold.
    • These increases were observed within 1 day and persisted for 2 weeks.
    • In vitro studies confirmed spontaneous increases in Gs alpha and G beta during organ culture.
    • Constant light-induced increases were blocked by isoproterenol administration.

    Conclusions:

    • Stimulus deprivation significantly increases both Gs alpha and G beta subunits in the pineal gland.
    • Adrenergic stimulation appears to regulate the levels of Gs alpha and G beta.
    • Findings contribute to understanding denervation supersensitivity at a molecular level.