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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

The cellular nature of genetic susceptibility to a virus

M KANTOCH, A WARWICK, F B BANG

    The Journal of Experimental Medicine
    |May 1, 1963
    PubMed

    Insights

    Mouse hepatitis virus (MHV) susceptibility in mice is influenced by genetics. Resistant mouse macrophages can temporarily become susceptible after exposure to a heat-stable substance within a large particle.

    Area of Science:

    • Immunology
    • Virology
    • Genetics

    Background:

    • Mouse hepatitis virus (MHV) is a significant pathogen in mouse models.
    • Understanding host genetic factors influencing MHV susceptibility is crucial for research.

    Purpose of the Study:

    • To investigate the genetic basis of mouse susceptibility to mouse hepatitis virus (MHV).
    • To establish a correlation between in vivo and in vitro susceptibility to MHV.

    Main Methods:

    • Peritoneal macrophage cultures from susceptible (PRI) and resistant (C3H) mice were used.
    • F1 and back-cross generations of mice were generated and tested for MHV susceptibility.
    • In vitro macrophage cultures were assessed for virus-induced degeneration.
    • In vivo susceptibility was determined through direct testing of mice.

    Main Results:

    • PRI mice and their macrophages were susceptible to MHV, while C3H mice and macrophages were resistant.
    • F1 macrophages showed mixed susceptibility, with segregation observed in back-cross generations.
    • A correlation between in vivo and in vitro MHV susceptibility was established.
    • Resistant macrophages temporarily acquired susceptibility after ingesting a particle containing a heat-stable substance.

    Conclusions:

    • MHV susceptibility in mice appears to be controlled by a single, incompletely expressed dominant gene.
    • In vitro macrophage susceptibility accurately reflects in vivo susceptibility to MHV.
    • Environmental factors can temporarily alter macrophage susceptibility to MHV.
    Keywords:
    HEPATITIS VIRUSESHEPATITIS, ANIMALMACROPHAGESTISSUE CULTURE

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