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Isolation of dengue type 3 from mosquitoes in Rangoon
Abstract:
A virus was isolated in suckling mice from Aedes aegypti collected from Rangoon area, during the month of July 1969. The virus was identified as a member of Group B arbovirus dengue type 3, by means of its ability to agglutinate goose erythrocyte at pH 6.6, haemagglutination inhibition and complement fixation tests, and its growth characteristics in Aedes albopictus cell cultures. The isolated dengue type 3 virus differs slightly from the Indian dengue type 3 virus in its ability to produce haemagglutinin of high titer from the early mouse passage, variation in the pH for optimum haemagglutination and ability to produce cytopathic effect in Vero cell cultures.
Insights
A new dengue type 3 virus was isolated from mosquitoes in Rangoon. This arbovirus shows slight differences compared to Indian strains, particularly in hemagglutination and cell culture effects.
Area of Science:
- Virology
- Arbovirology
- Medical Entomology
Background:
- Arboviruses, including dengue virus, pose significant public health threats.
- Mosquitoes, such as Aedes aegypti, are primary vectors for arboviruses.
- Dengue virus has multiple serotypes, necessitating ongoing surveillance and characterization.
Purpose of the Study:
- To isolate and identify arboviruses from mosquito vectors in the Rangoon area.
- To characterize the isolated dengue type 3 virus and compare it with existing strains.
- To understand the genetic and antigenic variations within dengue virus serotypes.
Main Methods:
- Virus isolation from Aedes aegypti mosquitoes in suckling mice.
- Serological assays including hemagglutination inhibition (HI) and complement fixation (CF) tests.
- Characterization of viral growth in Aedes albopictus cell cultures and cytopathic effects in Vero cell cultures.
Main Results:
- A Group B arbovirus, identified as dengue type 3, was successfully isolated.
- The isolated virus demonstrated specific hemagglutination properties at pH 6.6.
- Distinct differences were observed when compared to the Indian dengue type 3 strain, including early high-titer hemagglutinin production, optimal pH variation, and cytopathic effects in Vero cells.
Conclusions:
- The study successfully identified a dengue type 3 virus strain from Rangoon, Myanmar.
- The identified strain exhibits unique characteristics differentiating it from previously documented dengue type 3 viruses.
- These findings highlight the importance of continuous arbovirus surveillance and characterization for public health preparedness.