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Updated: Jun 18, 2026

12:40
High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 22, 2014
MALATE DEHYDROGENASE: MULTIPLE FORMS IN SEPARATED BLASTOMERES OF SEA URCHIN EMBRYOS
Abstract:
Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.
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